【文獻(xiàn)標(biāo)題】Sodium butyrate improved intestinal barrier in rabbits

【作者】Chenyang Li , Xiaoyang Chen , Bin Zhang , et.al

【作者單位】山東農(nóng)業(yè)大學(xué)（Shandong Agricultural University）

【文獻(xiàn)中引用產(chǎn)品】

兔子白介素4(IL-4)ELISA試劑盒

兔子γ干擾素(IFN-γ)ELISA試劑盒

兔分泌型免疫球蛋白A(sIgA)ELISA試劑盒

【關(guān)鍵詞】Rabbit; sodium butyrate;intestinal development;intestinal flora; intestinal;immune barrier

【DOI】doi.org/10.1080/1828051X.2020.1847209

【影響因子(IF)】2.17

【出版期刊】《Italian Journal of Animal Science》

【產(chǎn)品原文引用】

Analysis of intestinal immune barrier

After the rabbits were slaughtered, the jejunum, ileum and duodenum were placed in 1.5 mL cryopreservation tubes, flash-frozen in liquid nitrogen, and then preserved at 80 C until analysis. The contents of interleukin-4 (IL-4), interferon-gamma (IFN-c) and secretory immunoglobulin A (SIgA) in the jejunum, ileum and duodenum were measured in samples from each group. Five samples were randomly selected from each group, and approximately 1 g tissue was used for each sample. The tissue was homogenised, and then PBS was added to the homogenate at 1:9, then it was serially diluted into five concentrations. Each sample was assayed in duplicate. The tests were carried out according to the instructions of the corresponding kit, and the OD value was determined by absorbance at 450 nm. The contents of IL-4, IFN- c and SIgA were determined by enzyme linked immunosorbent assays (ELISA) (kits provided by Shanghai Hengyuan Biological Co., Ltd. Shanghai, China).

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