【文獻標題】Prediction and validation of monoclonal antibodies separation in aqueous two-phase system using molecular dynamic simulation 【作者】Yibo Guoa, Xi Chena, Xue Yub,et.al 【作者單位】華東科技大學(xué)（East China University of Science and Technology） 【文獻中引用產(chǎn)品】 人免疫球蛋白G(IgG)ELISA試劑盒 【關(guān)鍵詞】Monoclonal antibodies,Aqueous two-phase system,Molecular dynamic simulation,Prediction model 【DOI】doi.org/10.1016/j.chroma.2023.463921 【影響因子(IF)】4.6 【出版期刊】《Journal of Chromatography A》 【產(chǎn)品原文引用】 Na2SO4, K2SO4, sodium citrate, citric acid, sodium acetate,acetic acid, NaOH, HCl, Na2HPO4, NaH2PO4 and KH2PO4 were purchased from China National Pharmaceutical Industry Corporation Ltd. (Shanghai, China). NaCl and glycine were purchased from General-Reagent (Shanghai, China). EO20PO80 (3000 g/mol) was purchased from Guangchi Chemical Company (Taian, China). The size exclusion chromatography (SEC) column BioBasic SEC-300 (300×4.6 mm) was purchased from Thermo Fisher Scientific (Waltham, America). The amino acid sequences of rituximab and trastuzumab were obtained from Drugbank [25] and mAbs were constructed by others in our lab with purity more than 98%. Enzyme linked immunosorbent assay (ELISA) reagent test kit for human IgG was purchased from Shanghai Hengyuan Biological Technology co., LTD (Shanghai, China).
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